The authors demonstrate that glycogen is present in peripheral nerve, primarily in myelinating Schwann cells, and supports conduction in the large diameter, myelinated axon during periods of aglycemia. They used lactate biosensors to show that lactate is produced from the breakdown of glycogen in peripheral nerve and is passed to myelinated axons to support function during aglycemia.
Brown AM, Evans RD, Black J, Rans ... Read More
Another important breakthrough using Sarissa's biosensors. In a new paper in J Neurosci, Dr Phil Haydon's group have used sarissaprobe adenosine and inosine biosensors to demonstrate the important role of astrocytes in the diurnal control of adenosine release and the release of adenosine in response to sleep deprivation:
Schmitt LI, Sims RE, Dale N, Haydon PG. (2012) Wakefulness
affects synaptic and network activity by
This is the first paper to feature the adenosine biosensors fabricated on a carbon fibre electrode. The very small dimensions of these biosensors were essential for measuring adenosine levels inside islets -the first time this has been achieved. These direct measurements showed unexpectedly that adenosine release is inversely related to glucose concentration and does not arise from previous release of ATP in the isolated islets.
Ya ... Read More
In this paper sarissaprobe Ado biosensors were used to demonstrate direct action potential dependent release of adenosine from parallel fibres in the cerebellum. The authours used a mouse line lacking a key extracellular enzyme that converts ATP to adenosine to demosntrate that this was direct release of adenosine rather than adenosine arising from previously released ATP. Furthermore this direct release of adenosine was blocked by bafilomyc ... Read More
Adenosine sarissaprobe biosensors feature in the following recently published papers:
Wang W, Liu X, Schneider B, Zverina EA, Rus K, Wijeyesakere SJ, Fierke CA, Richardson RJ, Philbert MA (2012) Mixed Inhibition of Adenosine Deaminase Activity by 1,3-Dinitrobenzene: A Model for Understanding Cell-Selective Neurotoxicity in Chemically-Induced Energy Deprivation Syndromes in Brain. Read More