General instructions for all sarissaprobe® sensors
You can download specific instructions for all of our
sensors from the product pages.
Store in Fridge at 2-8 C before use. Operation of sensor
after use-by date is not guaranteed.
Prepare Buffer A: containing 10 mM NaPi buffer, pH 7.4, 100 mM
NaCl, 1 mM MgCl2, 2 mM glycerol. Pour a suitable volume
of Buffer A into rehydration pot, and immerse the tip of the sensor
into the buffer. Leave for at least 10 min.
This will improve sensor sensitivity by up to 3 fold. Cycle
the sensor from -500 mV to + 500 mV and back at a rate of 100 mV/s
for 10 cycles. Then polarize to +500 mV for calibration.
First calibration and sensor test
To test against the specific analyte, place sensor and Ag/AgCl
reference electrode in the bath, connect Ag/AgCl reference
electrode and sensor to 2-electrode potentiostat and polarize to
+500 mV. Allow sensor current to decay to an asymptote -take zero
reading then expose the sensor to analyte.
Typically test with 10 μM serotonin, 10 μM dopamine and 100 μM
Calibration during experiments
Regular calibration during an experiment is necessary to
monitor for possible loss of sensor sensitivity after contact with
tissue. Loss of sensor activity can be partially restored by
cycling as described above. Sensors should be calibrated out of
contact with tissue -as the tissue impedes free access of exogenous
analyte to the sensor surface. If the tissue is in a flow chamber,
the simplest procedure is to withdraw the sensor from the tissue
and allow a test solution of analyte (e.g. 10 µM to flow
Note that calibration of the sensor in a physiological buffer
may differ from that recorded in Buffer A due to the different
ionic composition of the buffers. The sensor should always be
calibrated in a saline of the composition use in the experiment